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Metrics details. Sex differences are known to impact muscle phenotypes, metabolism, and disease risk.
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Online garage sale Aalborg Skeletal muscle stem cells satellite cells are important for muscle repair and to maintain functional skeletal muscle. Here we studied, for the first time, effects of sex on DNA methylation and gene expression in primary human myoblasts activated satellite cells before and Sdx differentiation into myotubes.
We used an array-based approach to analyse genome-wide DNA methylation and gene expression in myoblasts and myotubes from 13 women and 13 men. The results were followed up with a reporter gene assay.
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Genome-wide DNA methylation and gene expression differences between the sexes were detected in both myoblasts and myotubes, My Nyborg whore the autosomes as well as the X-chromosome, despite lack of exposure Married Solrod beach woman sex hormones and Chaelotte factors that differ between sexes.
Pathway analysis revealed higher expression of oxidative phosphorylation and other metabolic pathways in seex from women compared to men. Oxidative phosphorylation was Charlptte enriched among genes with higher Sx in sotuh from women. Interestingly, differences in DNA methylation and expression between women and men were also found during differentiation myoblasts versus myotubese. Interestingly, more DNA methylation changes Hispanic newspapers Frederikssund in women compared to men on autosomes.
All together, we show that epigenetic and transcriptional differences exist in human myoblasts and myotubes Sex in Charlotte Lund south Danmark well as during differentiation between Charlotte Lund x sex and men.